Comprehensive Analysis of Antibody-Based Conjugates

Antibody Drug Conjugates (ADCs) and Antibody Oligonucleotide Conjugates (AOCs) are complex therapeutic modalities requiring precise analytical methods to ensure safety, efficacy, and regulatory compliance. Our specialized characterization services deliver high-resolution data for identity, conjugation ratio, and structural integrity across development stages.

1. Identity & Post-Translational Modifications (PTMs)

Techniques: Intact Mass Spectrometry, Peptide Mapping
We confirm the molecular identity and detect site-specific or global PTMs with high sensitivity.

• Verification of expected molecular weight (intact protein analysis)

• Detailed peptide mapping for amino acid sequence coverage

• Detection of glycosylation, oxidation, deamidation, and other critical modifications

2. Drug-to-Antibody Ratio (DAR) / Oligonucleotide-to-Antibody Ratio (OAR)

Techniques: Hydrophobic Interaction Chromatography (HIC), Anion Exchange Chromatography (AEX), UV-MS
Quantify the average number of payloads per antibody molecule to assess product consistency and potency.

• DAR/OAR profiling for batch comparability

• Detection of unconjugated species

• Support for release and stability testing

3. Biophysical Characterization

Techniques: Analytical Ultracentrifugation (AUC), Differential Scanning Calorimetry (DSC), Circular Dichroism (CD)
Assess the structural integrity and conformational stability of your ADC/AOC.

• Aggregation and heterogeneity analysis via AUC

• Thermal stability profiling with DSC

• Secondary structure confirmation with CD spectroscopy

Why Choose Us

• GLP/GMP-capable facilities

• Rapid turnaround and custom method development

• Expert support for IND, BLA, and regulatory submissions

• Scalable services from early discovery to clinical phases