Axela Biosciences provides advanced PhosphoFlow cytometry services for sensitive, single-cell analysis of intracellular signaling pathways in immune and cancer cells. Their assays are designed to support drug discovery, translational research, immuno-oncology, autoimmune disease studies, and biomarker development.

Overview

PhosphoFlow cytometry enables detection of protein phosphorylation events at the cellular level using phospho-specific antibodies and multiparameter flow cytometry. Unlike traditional bulk assays such as Western blotting, PhosphoFlow allows researchers to analyze signaling activity within specific cell populations in heterogeneous samples.

Axela’s platform supports simultaneous analysis of:

Cell surface markers
Intracellular phosphoproteins
Immune cell subsets
Signaling pathway activation states

This approach provides high-resolution insight into cellular responses, drug mechanism of action, pathway modulation, and immune activation.

Core Capabilities

Axela Biosciences offers:

Multiplex phosphoprotein profiling
Customized phospho-panel development
Immune and cancer cell signaling analysis
Single-cell pathway quantification
Drug response and target engagement studies
Biomarker discovery and translational assays
Multi-color flow cytometry and cell sorting support

Their assays are optimized across multiple sample and cell types, including PBMCs, tumor cells, primary immune cells, and preclinical samples.

Platform & Technical Expertise

Axela utilizes advanced flow cytometry infrastructure including CytoFlex S systems with 4-laser, 13-color capability for high-content cellular analysis. Their scientific team brings expertise in immunology, cytometry, bioanalysis, and translational biology to support complex translational research workflows.

Applications

PhosphoFlow services are commonly used for:

Immuno-oncology research
Cytokine signaling analysis
T-cell and NK-cell activation studies
Kinase pathway profiling
Drug candidate screening
Immune monitoring
Autoimmune disease research
Mechanism-of-action studies
Why Work With Axela Biosciences?

Axela Biosciences focuses on generating high-quality, information-rich data from limited or low-volume samples through ultrasensitive and multiplex assay platforms. Their workflows are tailored to project-specific goals, enabling rapid turnaround times, flexible study design, and scalable support for biotech, pharma, and academic research programs.

Request a Quote

Need support for phosphoprotein profiling, immune signaling analysis, or custom PhosphoFlow assay development?

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