Our Flow Cytometry Validation Services

We offer a multi-parameter approach to rigorously assess your antibody’s performance for flow cytometry, providing you with a complete characterization profile. Our antibody validation services can be used for:

• Unconjugated Primary Antibodies
• Directly Conjugated (DC) Antibodies
• Secondary Antibodies

Core Validation Assays:

• Optimal Titer Determination: We perform comprehensive titration analysis on relevant cell types to identify the ideal antibody concentration that maximizes the signal-to-noise ratio, preventing background noise and conserving your valuable reagent.
• Specificity Confirmation: We test whether your antibody binds to the correct target using a variety of robust methods.
• Positive & Negative Cell Lines: Staining of cell lines known to express or lack the target of interest provides clear evidence of specificity.
• Knockout (KO) Validation: As the gold standard, we use CRISPR-generated knockout cell lines to definitively demonstrate that the signal is absent without the target protein.
• Isotype Controls: We establish baseline fluorescence and assess non-specific Fc receptor binding using appropriate isotype controls.
• Performance on Primary Cells: We can test and verify antibody performance on specific primary cell populations (CD4+ T cells, B cells, monocytes, etc.) to ensure it works in your experimental system.
• Intracellular Staining Validation: We optimize and validate antibodies for intracellular targets, including verification of the best fixation and permeabilization protocol.