Service Description

Advance your tissue-based research with Akoya-certified spatial biology services powered by the PhenoCycler-Fusion platform.

This end-to-end service enables high-plex protein profiling across intact tissue sections while preserving the spatial relationships between cells, biomarkers, and tissue structures. Researchers can characterize complex cellular environments using multiplex panels containing 10 to 60 protein markers.

Services are available for FFPE and frozen tissue samples, including human, mouse, tissue microarray, and other preclinical specimens. Support is available from panel design and sample preparation through imaging, quality control, data delivery, and optional expert pathology review.

Key Capabilities

• Akoya-certified PhenoCycler-Fusion spatial biology workflow
• High-plex protein profiling with 10–60 markers
• Validated and customizable antibody panels
• FFPE and frozen tissue compatibility
• Human, mouse, tissue microarray, and custom animal-model samples
• High-resolution multiplex immunofluorescence imaging
• Quality-controlled, analysis-ready spatial imaging data
• Secure digital slide and data delivery
• Optional review by board-certified MD or DVM pathologists
• Support for both exploratory and translational research programs

Applications

Immuno-Oncology

Characterize the tumor microenvironment, quantify immune-cell infiltration, identify cellular neighborhoods, and evaluate spatial relationships between tumor and immune populations.

Biomarker Discovery

Assess the expression and spatial distribution of therapeutic targets, pharmacodynamic biomarkers, and disease-associated proteins within intact tissue.

Neuroscience

Map neuronal, glial, vascular, and immune-cell populations while preserving their organization within complex brain tissue.

Autoimmune and Inflammatory Disease

Evaluate tissue architecture, immune activation, inflammatory-cell recruitment, and localized biomarker expression.

Preclinical and Translational Research

Compare treatment groups, characterize disease models, evaluate mechanisms of action, and generate spatial evidence supporting therapeutic development.

Service Workflow

1. Project Consultation and Panel Design

Provide information about your tissue type, species, disease area, study objectives, and biomarkers of interest. The scientific team will help determine whether an existing panel or a customized marker panel is the best fit for your project.

2. Sample Submission

Submit FFPE blocks, unstained slides, frozen sections, tissue microarrays, or other compatible tissue samples. Shipping and sample-handling guidance can be provided before project initiation.

3. Assay Execution and Imaging

Samples are processed using an Akoya PhenoCycler-Fusion workflow. Multiplex staining, imaging, and quality-control procedures are performed to generate high-resolution spatial protein data.

4. Data Quality Review

Image quality, tissue integrity, staining performance, and marker-specific results are reviewed before delivery. Optional pathology review may be added when scientific interpretation or study reporting is required.

5. Digital Data Delivery

Clients receive secure access to high-resolution, analysis-ready spatial imaging files and associated project deliverables.

Why Choose This Service?

• Preserve spatial context while measuring dozens of protein biomarkers
• Reduce the need for serial tissue sections and repeated single-marker assays
• Access experienced spatial biology and histopathology support
• Develop customized panels aligned with specific research objectives
• Generate reproducible data suitable for downstream image analysis
• Integrate optional pathology expertise into study interpretation
• Support projects from early discovery through translational development

Sample Types Supported

• FFPE tissue blocks
• FFPE tissue sections
• Frozen tissue sections
• Tissue microarrays
• Human tissue
• Mouse tissue
• Other preclinical animal-model tissue, subject to project review

Deliverables

Project deliverables may include:

• High-resolution multiplex tissue images
• Analysis-ready QPTIFF image files
• Marker and panel documentation
• Quality-control summary
• Secure digital slide access
• Annotated images, when included in the project scope
• Optional board-certified pathologist review
• Optional study interpretation or pathology reporting